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Fermentative Hydrogen Production by Enterobacter sp. KTSMBNL-01 Isolated from Municipal Sewage Sludge: Optimization Studies
IJCA Proceedings on National Conference cum Workshop on Bioinformatics and Computational Biology
© 2014 by IJCA Journal
NCWBCB - Number 2
Year of Publication: 2014
Pugazhendhi A, Prakash P, Poornima Priyadharsani T.k, Valsala H and Thamaraiselvi K. Article: Fermentative Hydrogen Production by Enterobacter sp. KTSMBNL-01 Isolated from Municipal Sewage Sludge: Optimization Studies. IJCA Proceedings on National Conference cum Workshop on Bioinformatics and Computational Biology NCWBCB(2):25-28, May 2014. Full text available. BibTeX
@article{key:article,
author = {Pugazhendhi A and Prakash P and Poornima Priyadharsani T.k and Valsala H and Thamaraiselvi K},
title = {Article: Fermentative Hydrogen Production by Enterobacter sp. KTSMBNL-01 Isolated from Municipal Sewage Sludge: Optimization Studies},
journal = {IJCA Proceedings on National Conference cum Workshop on Bioinformatics and Computational Biology},
year = {2014},
volume = {NCWBCB},
number = {2},
pages = {25-28},
month = {May},
note = {Full text available}
}
Abstract
A fermentative hydrogen-producing strain KTSMBNL-01 was isolated from the sewage sludge and identified as Enterobacter sp. on the basis of the biochemical characteristics and 16S rRNA gene analysis. The optimization of batch conditions for the production of hydrogen by Enterobacter sp. was investigated. Various parameters namely initial pH, temperature and substrate concentrations were varied for hydrogen production. Maximum hydrogen yield (0. 86 mol/mol G) and cell growth (1. 59 g/L) was obtained at pH 7. 0 and at 37 °C. The strain was capable of producing hydrogen in the presence glucose, fructose, xylose, maltose, lactose, cellulose and starch, where the best results revealed with glucose. This study showed that Enterobacter sp. can efficiently produce H2 and it is a one more model microorganism for biohydrogen production.
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